Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)

SKU: MA1085

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Chicken,Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.25-0.5μg/ml, Human, mouse, rat, chicken

Gene Name: AKT1

Specificity: No cross reactivity with other proteins.

Background: PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1 (ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.

Immunogen: Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Staal, S. P.; Huebner, K.; Croce, C. M.; Parsa, N. Z.; Testa, J. R. : The AKT1 proto-oncogene maps to human chromosome 14, band q32. Genomics 2: 96-98, 1988. 2. Ackah, E.; Yu, J.; Zoellner, S.; Iwakiri, Y.; Skurk, C.; Shibata, R.; Ouchi, N.; Easton, R. M.; Galasso, G.; Birnbaum, M. J.; Walsh, K.; Sessa, W. C. : Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. J. Clin. Invest. 115: 2119-2127, 2005. 3. Chen, J.; Somanath, P. R.; Razorenova, O.; Chen, W. S.; Hay, N.; Bornstein, P.; Byzova, T. V. : Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo. Nature Med. 11: 1188-1196, 2005.

Uniprot ID: P47196

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: PKB-175

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight: 55735 MW

Gene ID: Human(207), Mouse(11651), Rat(24185)

Protein Name: RAC-alpha serine/threonine-protein kinase

Gene Full Name: AKT serine/threonine kinase 1

Synonyms: AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; ONCOGENE AKT1; PROTEIN KINASE B-ALPHA; RAC SERINE/THREONINE PROTEIN KINASE; Protein kinase B; Proto-oncogene c-Akt; RAC-PK-alpha