Anti-PIK3C2B Antibody Picoband®

SKU: A06732-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse
ELISA, 0.1-0.5 μg/ml

Gene Name: PIK3C2B

Specificity:

Background: The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner.

Immunogen: E.coli-derived human PIK3C2B recombinant protein (Position: R21-E1009).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Brown, R. A., Ho, L. K. F., Weber-Hall, S. J., Shipley, J. M., Fry, M. J. Identification and cDNA cloning of a novel mammalian C2 domain-containing phosphoinositide 3-kinase, HsC2-PI3K. Biochem. Biophys. Res. Commun. 233: 537-544, 1997. 2. Maehama, T., Fukasawa, M., Date, T., Wakita, T., Hanada, K. A class II phosphoinositide 3-kinase plays an indispensable role in hepatitis C virus replication. Biochem. Biophys. Res. Commun. 440: 150-156, 2013. 3. Maffucci, T., Cooke, F. T., Foster, F. M., Traer, C. J., Fry, M. J., Falasca, M. Class II phosphoinositide 3-kinase defines a novel signaling pathway in cell migration. J. Cell Biol. 169: 789-799, 2005.

Uniprot ID: O00750

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 160-180 kDa

Calculated Molecular Weight: 185 kDa

Gene ID: 5287

Protein Name: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta

Gene Full Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta

Synonyms: C2 PI3K; PI3K C2 beta; PIK3C2B