Anti-PIGV Antibody Picoband®

SKU: A08698-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PIGV

Specificity:

Background: GPI mannosyltransferase 2 is an enzyme that in humans is encoded by the PIGV gene. This gene encodes a mannosyltransferase enzyme involved in the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is a complex glycolipid that functions as a membrane anchor for many proteins and plays a role in multiple cellular processes including protein sorting and signal transduction. The encoded protein is localized to the endoplasmic reticulum and transfers the second mannose to the GPI backbone. Mutations in this gene are associated with hyperphosphatasia cognitive disability syndrome. Alternatively spliced transcript variants have been observed for this gene.

Immunogen: E.coli-derived human PIGV recombinant protein (Position: Q26-Y470).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Horn, D., Krawitz, P., Mannhardt, A., Korenke, G. C., Meinecke, P. Hyperphosphatasia-mental retardation syndrome due to PIGV mutations: expanded clinical spectrum. Am. J. Med. Genet. 155A: 1917-1922, 2011. 2. Kang, J. Y. Hong, Y., Ashida, H., Shishioh, N., Murakami, Y., Morita, Y. S., Maeda, Y., Kinoshita, T. PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol. J. Biol. Chem. 280: 9489-9497, 2005. 3. Krawitz, P. M., Schweiger, M. R., Rodelsperger, C., Marcelis, C., Kolsch, U., Meisel, C., Stephani, F., Kinoshita, T., Murakami, Y., Bauer, S., Isau, M., Fischer, A., and 17 others. Identity-by-descent filtering of exome sequence data identifies PIGV mutations in hyperphosphatasia mental retardation syndrome. Nature Genet. 42: 827-829, 2010.

Uniprot ID: Q9NUD9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight:

Gene ID: 55650

Protein Name: GPI alpha-1,6-mannosyltransferase 2

Gene Full Name: phosphatidylinositol glycan anchor biosynthesis class V

Synonyms: PIGV; GPI mannosyltransferase 2; GPI mannosyltransferase II; GPI-MT-II; Phosphatidylinositol-glycan biosynthesis class V protein; PIG-V