Anti-PIF1 Antibody Picoband®

SKU: A02315-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: PIF1

Specificity:

Background: This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus.

Immunogen: E.coli-derived human PIF1 recombinant protein (Position: Q31-R618). Human PIF1 shares 87.6% and 87.8% amino acid (aa) sequence identity with mouse and rat PIF1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Mateyak, M. K., Zakian, V. A. Human PIF helicase is cell cycle regulated and associates with telomerase. Cell Cycle 5: 2796-2804, 2006. 2. Paeschke, K., Bochman, M. L., Garcia, P. D., Cejka, P., Friedman, K. L., Kowalczykowski, S. C., Zakian, V. A. Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature 497: 458-462, 2013. 3. Zhang, D.-H., Zhou, B., Huang, Y., Xu, L.-X., Zhou, J.-Q. The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity. Nucleic Acids Res. 34: 1393-1404, 2006.

Uniprot ID: Q9H611

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 77 kDa

Calculated Molecular Weight: 77 kDa

Gene ID: 80119

Protein Name: ATP-dependent DNA helicase PIF1

Gene Full Name: PIF1 5'-to-3' DNA helicase

Synonyms: PIF1; C15orf20; ATP-dependent DNA helicase PIF1; EC 3.6.4.12; DNA repair and recombination helicase PIF1; PIF1/RRM3 DNA helicase-like protein