Anti-PI3K-gamma/PIK3CG Antibody Picoband®

SKU: A01517-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: PIK3CG

Specificity:

Background: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform is an enzyme that in humans is encoded by the PIK3CG gene. Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene.

Immunogen: E.coli-derived human PI3K-gamma/PIK3CG recombinant protein (Position: K52-D308).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Anzelon, A. N., Wu, H., Rickert, R. C. Pten inactivation alters peripheral B lymphocyte fate and reconstitutes CD19 function. Nature Immun. 4: 287-294, 2003. 2. Barber, D. F., Bartolome, A., Hernandez, C., Flores, J. M., Redondo, C., Fernandez-Arias, C., Camps, M., Ruckle, T., Schwarz, M. K., Rodriguez, S., Martinez-A, C., Balomenos, D., Rommel, C., Carrera, A. C. PI3K-gamma inhibition blocks glomerulonephritis and extends lifespan in a mouse model of systemic lupus. Nature Med. 11: 933-935, 2005. 3. Barbier, M., Attoub, S., Calvez, R., Laffargue, M., Jarry, A., Mareel, M., Altruda, F., Gespach, C., Wu, D., Lu, B., Hirsch, E., Wymann, M. P. Weakening link to colorectal cancer? Nature 413: 796 only, 2001.

Uniprot ID: P48736

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 126 kDa

Calculated Molecular Weight:

Gene ID: 5294

Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform

Gene Full Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma

Synonyms: p120 PI3K; PI3 Kinase p110 Gamma; PI3 kinase subunit gamma; PI3CG; PI3K; PI3K gamma; PI3K p110(gamma); PI3Kgamma; PIK3; PIK3CG; PtdIns 3 kinase subunit gamma