Anti-PDX1 Antibody Picoband®

SKU: A00491-4

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PDX1

Specificity:

Background: The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (IDDM), as well as maturity onset diabetes of the young type 4 (MODY4).

Immunogen: E.coli-derived human PDX1 recombinant protein (Position: M1-E145). Human PDX1 shares 87.7% and 86.9% amino acid (aa) sequence identity with mouse and rat PDX1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Cockburn, B. N., Bermano, G., Boodram, L.-L. G., Teelucksingh, S., Tsuchiya, T., Mahabir, D., Allan, A. B., Stein, R., Docherty, K., Bell, G. I. Insulin promoter factor-1 mutations and diabetes in Trinidad: identification of a novel diabetes-associated mutation (E224K) in an Indo-Trinidadian family. J. Clin. Endocr. Metab. 89: 971-978, 2004. 2. Fajans, S. S., Bell, G. I., Paz, V. P., Below, J. E., Cox, N. J., Martin, C., Thomas, I. H., Chen, M. Obesity and hyperinsulinemia in a family with pancreatic agenesis and MODY caused by the IPF1 mutation Pro63fsX60. Transl. Res. 156: 7-14, 2010. 3. Fiedorek, F. T., Jr., Kay, E. S. Mapping of the insulin promoter factor 1 gene (Ipf1) to distal mouse chromosome 5. Genomics 28: 581-584, 1995.

Uniprot ID: P52945

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 40 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 3651

Protein Name: Pancreas/duodenum homeobox protein 1

Gene Full Name: pancreatic and duodenal homeobox 1

Synonyms: Glucose sensitive factor; GSF; IDX 1; IPF 1; IPF1; Islet/duodenum homeobox 1; IUF 1; IUF1; MODY4; PDX 1; PDX1; STF 1