Anti-PDLIM4 Antibody Picoband®

SKU: A09573-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PDLIM4

Specificity:

Background: PDZ and LIM domain protein 4 is a protein that in humans is encoded by the PDLIM4 gene. RIL, also known as PDLIM4 is a putative tumor suppressor gene. RIL is transcriptionally repressed by hypermethylation in a variety of tumors including breast and prostate. The difference in methylation status of RIL between normal and cancerous tissues is being investigated as to determine the use of RIL as a potential biomarker. Two isoforms of human RIL one have been identified.

Immunogen: E.coli-derived human PDLIM4 recombinant protein (Position: Y137-D271). Human PDLIM4 shares 91.2% and 89% amino acid (aa) sequence identity with mouse and rat PDLIM4, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bashirova, A. A., Markelov, M. L., Shlykova, T. V., Levshenkova, E. V., Alibaeva, R. A., Frolova, E. I. The human RIL gene: mapping to human chromosome 5q31.1, genomic organization and alternative transcripts. Gene 210: 239-245, 1998. 2. Boumber, Y. A., Kondo, Y., Chen, X., Shen, L., Gharibyan, V., Konishi, K., Estey, E., Kantarjian, H., Garcia-Manero, G., Issa, J.-P. J. RIL, a LIM gene on 5q31, is silenced by methylation in cancer and sensitizes cancer cells to apoptosis. Cancer Res. 67: 1997-2005, 2007. 3. Kiess, M., Scharm, B., Aguzzi, A., Hajnal, A., Klemenz, R., Schwarte-Waldhoff, I., Schafer, R. Expression of ril, a novel LIM domain gene, is down-regulated in Hras-transformed cells and restored in phenotypic revertants. Oncogene 10: 61-68, 1995.

Uniprot ID: P50479

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 35,26 kDa

Gene ID: 8572

Protein Name: PDZ and LIM domain protein 4

Gene Full Name: PDZ and LIM domain 4

Synonyms: PDLIM4; RIL; PDZ and LIM domain protein 4; LIM protein RIL; Reversion-induced LIM protein