Anti-PAF1/PD2 Picoband® Antibody

SKU: A01640-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,ELISA

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: PAF1

Specificity: No cross reactivity with other proteins.

Background: PAF1 encodes a subunit of the polymerase associated factor (PAF1) complex. It is mapped to 19q13.2. The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen: E.coli-derived human PAF1/PD2 recombinant protein (Position: N18-D355).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Chen, F. X., Xie, P., Collings, C. K., Cao, K., Aoi, Y., Marshall, S. A., Rendleman, E. J., Ugarenko, M., Ozark, P. A., Zhang, A., Shiekhattar, R., Smith, E. R., Zhang, M. Q., Shilatifard, A. PAF1 regulation of promoter-proximal pause release via enhancer activation. Science 357: 1294-1298, 2017. 2. Marazzi, I., Ho, J. S. Y., Kim, J., Manicassamy, B., Dewell, S., Albrecht, R. A., Seibert, C. W., Schaefer, U., Jeffrey, K. L., Prinjha, R. K., Lee, K., Garcia-Sastre, A., Roeder, R. G., Tarakhovsky, A. Suppression of the antiviral response by an influenza histone mimic. Nature 483: 428-433, 2012. 3. Moniaux, N., Nemos, C., Schmied, B. M., Chauhan, S. C., Deb, S., Morikane, K., Choudhury, A., VanLith, M., Sutherlin, M., Sikela, J. M., Hollingsworth, M. A., Batra, S. K. The human homologue of the RNA polymerase II-associated factor 1 (hPaf1), localized on the 19q13 amplicon, is associated with tumorigenesis. Oncogene 25: 3247-3257, 2006.

Uniprot ID: Q8N7H5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 80 kDa

Calculated Molecular Weight: 141435 MW

Gene ID: 54623

Protein Name: RNA polymerase II-associated factor 1 homolog

Gene Full Name: PAF1 component of Paf1/RNA polymerase II complex

Synonyms: F23149_1; hPAF1; PAF1; PD2