Anti-OTULIN Antibody Picoband®

SKU: A07938-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: OTULIN

Specificity:

Background: This gene encodes a member of the peptidase C65 family of ubiquitin isopeptidases. Members of this family remove ubiquitin from proteins. The encoded enzyme specifically recognizes and removes M1 (Met1)-linked, or linear, ubiquitin chains from protein substrates. Linear ubiquitin chains are known to regulate the NF-kappa B signaling pathway in the context of immunity and inflammation. Mutations in this gene cause a potentially fatal autoinflammatory syndrome in human patients.

Immunogen: E.coli-derived human OTULIN recombinant protein (Position: A49-L352).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Damgaard, R. B., Walker, J. A., Marco-Casanova, P., Morgan, N. V., Titheradge, H. L., Elliott, P. R., McHale, D., Maher, E. R., McKenzie, A. N. J., Komander, D. The deubiquitinase OTULIN is an essential negative regulator of inflammation and autoimmunity. Cell 166: 1215-1230, 2016. 2. Gross, M. B. Personal Communication. Baltimore, Md. 3/25/2014. 3. Heger, K., Wickliffe, K. E., Ndoja, A., Zhang, J., Murthy, A., Dugger, D. L., Maltzman, A., de Sousa E Melo, F., Hung, J., Zeng, Y., Verschueren, E., Kirkpatrick, D. S., and 10 others. OTULIN limits cell death and inflammation by deubiquitinating LUBAC. Nature 559: 120-124, 2018.

Uniprot ID: Q96BN8

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 43-45 kDa

Calculated Molecular Weight: 49229 MW

Gene ID: 90268

Protein Name: Ubiquitin thioesterase otulin

Gene Full Name: OTU deubiquitinase with linear linkage specificity

Synonyms: OTU deubiquitinase with linear linkage specificity; AIPDS; FAM105B; GUM; OTULIN