Anti-NUP93 Antibody Picoband®

SKU: A07090-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC/IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: NUP93

Specificity:

Background: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene encodes a nucleoporin protein that localizes both to the basket of the pore and to the nuclear entry of the central gated channel of the pore. The encoded protein is a target of caspase cysteine proteases that play a central role in programmed cell death by apoptosis. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen: E.coli-derived human NUP93 recombinant protein (Position: M1-Q677). Human NUP93 shares 97.8% amino acid (aa) sequence identity with both mouse and rat NUP93.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Braun, D. A., Sadowski, C. E., Kohl, S., Lovric, S., Astrinidis, S. A., Pabst, W. L., Gee, H. Y., Ashraf, S., Lawson, J. A., Shril, S., Airik,, M., Tan, W., and 15 others. Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause steroid-resistant nephrotic syndrome. Nature Genet. 48: 457-465, 2016. 2. Grandi, P., Dang, T., Pane, N., Shevchenko, A., Mann, M., Forbes, D., Hurt, E. Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly. Molec. Biol. Cell 8: 2017-2038, 1997. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 9/15/2011.

Uniprot ID: Q8N1F7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 93 kDa

Calculated Molecular Weight: 93 kDa

Gene ID: 9688

Protein Name: Nuclear pore complex protein Nup93

Gene Full Name: nucleoporin 93

Synonyms: 93 kDa nucleoporin; KIAA0095; nucleoporin 93kDa; Nucleoporin Nup93; NUP93