Anti-NSMCE2 Antibody Picoband®

SKU: A06693-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IP,IF,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NSMCE2

Specificity:

Background: This gene encodes a member of a family of E3 small ubiquitin-related modifier (SUMO) ligases that mediates the attachment of a SUMO protein to proteins involved in nuclear transport, transcription, chromosome segregation and DNA repair. The encoded protein is part of the structural maintenance of chromosomes (SMC) 5/6 complex which plays a key role genome maintenance, facilitating chromosome segregation and suppressing mitotic recombination. A knockout of the orthologous mouse gene is lethal prior to embryonic day 10.5. Naturally occurring mutations in this gene, that abolish the SUMO ligase activity, are associated with primordial dwarfism and extreme insulin resistance.

Immunogen: E.coli-derived human NSMCE2 recombinant protein (Position: F14-D228).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gross, M. B. Personal Communication. Baltimore, Md. 12/8/2016. 2. Payne, F., Colnaghi, R., Rocha, N., Seth, A., Harris, J., Carpenter, G., Bottomley, W. E., Wheeler, E., Wong, S., Saudek, V., Savage, D., O'Rahilly, S., Carel, J.-C., Barroso, I., O'Driscoll, M., Semple, R. Hypomorphism in human NSMCE2 linked to primordial dwarfism and insulin resistance. J. Clin. Invest. 124: 4028-4038, 2014. 3. Potts, P. R., Yu, H. Human MMS21/NSE2 is a SUMO ligase required for DNA repair. Molec. Cell. Biol. 25: 7021-7032, 2005.

Uniprot ID: Q96MF7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 34 kDa

Calculated Molecular Weight: 104553 MW

Gene ID: 286053

Protein Name: E3 SUMO-protein ligase NSE2

Gene Full Name: NSE2 SUMO ligase component of SMC5/6 complex

Synonyms: C8orf36; E3 SUMO protein ligase NSE2; hMMS21; MMS21; MMS21 homolog; Non SMC element 2 homolog; NSE2; NSMCE2