Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2)

SKU: M03515

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunocytochemistry, 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Gene Name: NONO

Specificity: No cross reactivity with other proteins.

Background: Non-POU domain-containing octamer-binding protein is a protein that in humans is encoded by the NONO gene. This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human nmt55 p54nrb, identical to the related mouse and rat sequences.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: NONO Non-POU domain containing, octamer-binding. 2. Dong B, Horowitz DS, Kobayashi R, Krainer AR (Oct 1993). Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6. Nucleic Acids Res. 21 (17): 4085–92. 3. Traish AM, Huang YH, Ashba J, Pronovost M, Pavao M, McAneny DB, Moreland RB (Dec 1997). Loss of expression of a 55 kDa nuclear protein (nmt55) in estrogen receptor-negative human breast cancer. Diagn. Mol. Pathol. 6(4): 209–21.

Uniprot ID: Q15233

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: 11E2

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight:

Gene ID: 4841

Protein Name: Non-POU domain-containing octamer-binding protein

Gene Full Name: non-POU domain containing octamer binding

Synonyms: 55 kDa nuclear protein; NONO; NonO protein; NRB54; P54; p54(nrb); P54NRB