Anti-NHLRC2 Antibody Picoband®

SKU: A17232-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NHLRC2

Specificity:

Background: NHL Repeat Containing Protein 2, or NHLRC2, is a protein encoded by the NHLRC2 gene. NHLRC2 (NHL repeat containing 2) is a 726 amino acid protein that contains six NHL repeats and one thioredoxin domain. Existing as two alternatively spliced isoforms, NHLRC2 is encoded by a gene that maps to human chromosome 10q25.3. Spanning nearly 135 million base pairs, chromosome 10 makes up approximately 4.5% of total DNA in cells and encodes nearly 1,200 genes. Several protein-coding genes, including those that encode for chemokines, cadherins, excision repair proteins, early growth response factors (Egrs) and fibroblast growth receptors (FGFRs), are located on chromosome 10. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman’s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria.

Immunogen: E.coli-derived human NHLRC2 recombinant protein (Position: E67-Q588).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Biterova, E., Ignatyev, A., Uusimaa, J., Hinttala, R., Ruddock, L. W. Structural analysis of human NHLRC2, mutations of which are associated with FINCA disease. PLoS One 13: e0202391, 2018. Note: Electronic Article. 2. Brodsky, N. N., Boyarchuk, O., Kovalchuk, T., Hariyan, T., Rice, A., Ji, W., Khokha,, M., Lakhani, S., Lucas, C. L. Novel compound heterozygous variants in NHLRC2 in a patient with FINCA syndrome. J. Hum. Genet. 65: 911-915, 2020. 3. Gross, M. B. Personal Communication. Baltimore, Md. 1/17/2019.

Uniprot ID: Q8NBF2

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 79 kDa

Calculated Molecular Weight: 24145 MW

Gene ID: 374354

Protein Name: NHL repeat-containing protein 2

Gene Full Name: NHL repeat containing 2

Synonyms: DKFZp779F115; FLJ20147; FLJ25621; FLJ33312; NHL repeat containing 2; NHLRC2