Anti-NFS1 Antibody Picoband®

SKU: A05061-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NFS1

Specificity:

Background: Cysteine desulfurase, mitochondrial is an enzyme that in humans is encoded by the NFS1 gene. Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described.

Immunogen: E.coli-derived human NFS1 recombinant protein (Position: R107-L356). Human NFS1 shares 96.4% and 96% amino acid (aa) sequence identity with mouse and rat NFS1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Alvarez, S. W., Sviderskiy, V. O., Terzi, E. M., Papagiannakopoulos, T., Moreira, A. L., Adams, S., Sabatini, D. M., Birsoy, K., Possemato, R. NFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis. Nature 551: 639-643, 2017. Note: Erratum: Nature 609: E12, 2022. 2. Biederbick, A., Stehling, O., Rosser, R., Niggemeyer, B., Nakai, Y., Elsasser, H.-P., Lill, R. Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein biogenesis and iron regulation. Molec. Cell. Biol. 26: 5675-5687, 2006. 3. Farhan, S. M. K., Wang, J., Robinson, J. F., Lahiry P., Siu, V. M., Prasad, C., Kronick, J. B., Ramsay, D. A., Rupar C. A., Hegele, R. A. Exome sequencing identifies NFS1 deficiency in a novel Fe-S cluster disease, infantile mitochondrial complex II/III deficiency. Molec. Genet. Genomic Med. 2: 73-80, 2014.

Uniprot ID: Q9Y697

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 45 kDa

Calculated Molecular Weight:

Gene ID: 9054

Protein Name: Cysteine desulfurase

Gene Full Name: NFS1 cysteine desulfurase

Synonyms: HUSSY 08; IscS; NFS1; NIFS