Anti-NFAT5 Antibody Picoband®

SKU: A01815-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IF,IHC,ICC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: NFAT5

Specificity:

Background: The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human NFAT5 recombinant protein (Position: M1-D394).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Hebinck, A., Dalski, A., Engel, H., Mattei, M.-G., Hawken, R., Schwinger, E., Zuhlke, C. Assignment of transcription factor NFAT5 to human chromosome 16q22.1, murine chromosome 8D and porcine chromosome 6p1.4 and comparison of the polyglutamine domains. Cytogenet. Cell Genet. 90: 68-70, 2000. 2. Lopez-Rodriguez, C., Antos, C. L., Shelton, J. M., Richardson, J. A., Lin, F., Novobrantseva, T. I., Bronson, R. T., Igarashi, P., Rao, A., Olson, E. N. Loss of NFAT5 results in renal atrophy and lack of tonicity-responsive gene expression. Proc. Nat. Acad. Sci. 101: 2392-2397, 2004. 3. Lopez-Rodriguez, C., Aramburu, J., Rakeman, A. S., Rao, A. NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun. Proc. Nat. Acad. Sci. 96: 7214-7219, 1999.

Uniprot ID: O94916

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 220 kDa

Calculated Molecular Weight: 160 kDa

Gene ID: 10725

Protein Name: Nuclear factor of activated T-cells 5

Gene Full Name: nuclear factor of activated T cells 5

Synonyms: nuclear factor of activated T cells 5; NF-AT5; NFATL1; NFATZ; OREBP; TONEBP; NFAT5