Anti-NF-M/NEFM Antibody Picoband®

SKU: A06821-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunoprecipitation, 0.5-2 μg/ml, Human
Immunofluorescence, 5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: NEFM

Specificity:

Background: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Immunogen: E.coli-derived human NF-M/NEFM recombinant protein (Position: Q125-D916). Human NF-M/NEFMshares 72.3% and 72% amino acid (aa) sequence identity with mouse and rat NF-M/NEFM, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 5/21/2014. 2. Levy, E., Liem, R. K. H., D'Eustachio, P., Cowan, N. J. Structure and evolutionary origin of the gene encoding NF-M, the middle-molecular-mass neurofilament protein. Europ. J. Biochem. 166: 71-77, 1987. 3. Myers, M. W., Lazzarini, R. A., Lee, V. M.-Y., Schlaepfer, W. W., Nelson, D. L. The human mid-size neurofilament subunit: a repeated protein sequence and the relationship of its gene to the intermediate filament gene family. EMBO J. 6: 1617-1626, 1987.

Uniprot ID: P07197

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 160 kDa

Calculated Molecular Weight: 102 kDa

Gene ID: 4741

Protein Name: Neurofilament medium polypeptide

Gene Full Name: neurofilament medium chain

Synonyms: 160 kDa neurofilament protein; NEF3; NEFM; neurofilament; Neurofilament 3; NF; NF M; NFM; NF-M