Anti-NDUFAF1 Antibody Picoband®

SKU: A10145

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NDUFAF1

Specificity:

Background: Complex I intermediate-associated protein 30, mitochondrial (CIA30) is a protein that in humans is encoded by the NDUFAF1 gene. This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19.

Immunogen: E.coli-derived human NDUFAF1 recombinant protein (Position: D79-K327).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dunning, C. J. R., McKenzie, M., Sugiana, C., Lazarou, M., Silke, J., Connelly, A., Fletcher, J. M., Kirby, D. M., Thorburn, D. R., Ryan, M. T. Human CIA30 is involved in the early assembly of mitochondrial complex I and mutations in its gene cause disease. EMBO J. 26: 3227-3237, 2007. 2. Fassone, E., Taanman, J.-W., Hargreaves, I. P., Sebire, N. J., Cleary, M. A., Burch, M., Rahman, S. Mutations in the mitochondrial complex I assembly factor NDUFAF1 cause fatal infantile hypertrophic cardiomyopathy. J. Med. Genet. 48: 691-697, 2011. 3. Janssen, R., Smeitink, J., Smeets, R., van den Heuvel, L. CIA30 complex I assembly factor: a candidate for human complex I deficiency? Hum. Genet. 110: 264-270, 2002.

Uniprot ID: Q9Y375

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 36 kDa

Calculated Molecular Weight: 41409 MW

Gene ID: 51103

Protein Name: Complex I intermediate-associated protein 30, mitochondrial

Gene Full Name: NADH:ubiquinone oxidoreductase complex assembly factor 1

Synonyms: CGI 65; CGI65; CIA30; NDUFAF1