Anti-MSLNL Antibody Picoband®

SKU: A17693

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MSLNL

Specificity:

Background: Loss of gene function is implicated in the emergence of novel phenotypes during organism evolution. Human MSLNL has a nonsense mutation in exon 10 and two polymorphic mutations: a frameshift in exon 3 and a nonsense codon in exon 8. The gorilla gene also shows multiple deleterious mutations, including a premature stop codon, a deletion, and a splice site mutation. Molecular evolutionary analysis indicated relaxed selection pressure on MSLNL in African great ape lineages, which suggested that MSLNL might have become inactivated before the divergence of human, chimpanzee and gorilla. The mouse Mslnl gene is highly expressed in olfactory epithelium and moderately expressed in several other tissues. We propose that the loss of MSLNL may be associated with the evolution of the olfactory system in African great apes including human.

Immunogen: E.coli-derived human MSLNL recombinant protein (Position: Q65-H423).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. B, D. W. K. A. , C, S. J. , A, D. S. K. , D, H. S. K. , A, S. B. S. , & A, Y. H. . (2012). Inactivation of the mslnl gene encoding mesothelin-like protein during african great ape evolution. Gene, 496( 1), 17-21. 2. Hollevoet, K. , Nackaerts, K. , Gosselin, R. , Wever, W. D. , & Meerbeeck, J. P. V. . (2011). Soluble mesothelin, megakaryocyte potentiating factor, and osteopontin as markers of patient response and outcome in mesothelioma. Journal of Thoracic Oncology, 6(11), 1930-1937. 3. Daniels, R. J. , Peden, J. F. , Lloyd, C. , Horsley, S. W. , & Higgs, D. R. . (2001). Sequence, structure and pathology of the fully annotated terminal 2 mb of the short arm of human chromosome 16. Human Molecular Genetics, 10(4), 339-352.

Uniprot ID: Q96KJ4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 75 kDa

Calculated Molecular Weight: 24145 MW

Gene ID: 401827

Protein Name: Mesothelin-like protein

Gene Full Name: mesothelin like

Synonyms: MSLNL; C16orf37; MPFL