Anti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4)

SKU: M11793

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: PMPCB

Specificity:

Background: Mitochondrial-processing peptidase subunit beta is an enzyme that in humans is encoded by the PMPCB gene. This gene is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.

Immunogen: E.coli-derived human MPPB/PMPCB recombinant protein (Position: E23-Q479).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Fields, S., Song, O. A novel genetic system to detect protein-protein interactions. (Letter) Nature 340: 245-246, 1989. 2. Gordon, D. M., Shi, Q., Dancis, A., Pain, D. Maturation of frataxin within mammalian and yeast mitochondria: one-step processing by matrix processing peptidase. Hum. Molec. Genet. 8: 2255-2262, 1999. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/3/2018.

Uniprot ID: O75439

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 9F13E4

Observed Molecular Weight: 43 kDa

Calculated Molecular Weight:

Gene ID: 9512

Protein Name: Mitochondrial-processing peptidase subunit beta

Gene Full Name: peptidase, mitochondrial processing subunit beta

Synonyms: Beta MPP; MPP11; MPPB; MPPP52; P 52; PMPCB