Anti-MitoNEET/CISD1 Antibody Picoband®

SKU: A04360-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: CISD1

Specificity:

Background: CISD1 is a member of the CDGSH domain-containing family and may play a role in the regulation of mitochondrial oxidative capacity. This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2.

Immunogen: E.coli-derived human MitoNEET/CISD1 recombinant protein (Position: Y28-E93).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Colca, J. R., McDonald, W. G., Waldon, D. J., Leone, J. W., Lull, J. M., Bannow, C. A., Lund, E. T., Mathews, W. R. Identification of a novel mitochondrial protein ('mitoNEET') cross-linked specifically by a thiazolidinedione photoprobe. Am. J. Physiol. Endocr. Metab. 286: E252-60, 2004. 2. Hou, X., Liu, R., Ross, S., Smart, E. J., Zhu, H., Gong, W. Crystallographic studies of human MitoNEET. J. Biol. Chem. 282: 33242-33246, 2007. 3. Lin, J., Zhou, T., Ye, K., Wang, J. Crystal structure of human mitoNEET reveals distinct groups of iron-sulfur proteins. Proc. Nat. Acad. Sci. 104: 14640-14645, 2007.

Uniprot ID: Q9NZ45

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 15 kDa

Calculated Molecular Weight: 12 kDa

Gene ID: 55847

Protein Name: CDGSH iron-sulfur domain-containing protein 1

Gene Full Name: CDGSH iron sulfur domain 1

Synonyms: C10orf70; CDGSH iron sulfur domain 1; CISD1; MDS029; mitoNEET; Mnt; ZCD1