Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband® (monoclonal, 2D6)

SKU: M01065

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat

Gene Name: MUT

Specificity: No cross reactivity with other proteins.

Background: Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MUT, different from the related mouse sequence by one amino acid.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Adjalla, C. E., Hosack, A. R., Gilfix, B. M., Lamothe, E., Sun, S., Chan, A., Evans, S., Matiaszuk, N. V., Rosenblatt, D. S. Seven novel mutations in mut methylmalonic aciduria. Hum. Mutat. 11: 270-274, 1998. 2. Berger, I., Shaag, A., Anikster, Y., Baumgartner, E. R., Bar-Meir, M., Joseph, A., Elpeleg, O. N. Mutation analysis of the MCM gene in Israeli patients with mut(0) disease. Molec. Genet. Metab. 73: 107-110, 2001. 3. Cavicchi, C., Donati, M. A., Funghini, S., la Marca, G., Malvagia, S., Ciani, F., Poggi, G. M., Pasquini, E., Zammarchi, E., Morrone, A. Genetic and biochemical approach to early prenatal diagnosis in a family with mut methylmalonic aciduria. Clin. Genet. 69: 72-76, 2006.

Uniprot ID: P22033

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 2D6

Observed Molecular Weight: 83 kDa

Calculated Molecular Weight:

Gene ID: 4594

Protein Name: Methylmalonyl-CoA mutase, mitochondrial

Gene Full Name: methylmalonyl-CoA mutase

Synonyms: MCM; Methylmalonyl CoA isomerase; MUT