Anti-MELK Antibody Picoband®

SKU: A04038-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,IHC,ICC,WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MELK

Specificity:

Background: Maternal embryonic leucine zipper kinase (MELK) is an enzyme that in humans is encoded by the MELK gene. Maternal embryonic leucine-zipper kinase (MELK) is a key regulator of survival of stemlike GBM cells in vitro. MELK expression is increased in breast cancer tissue and this increase is also associated with poor patient survival, as predicted for a candidate oncogene.

Immunogen: E.coli-derived human MELK recombinant protein (Position: H66-Q629).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Heyer, B. S., Warsowe, J., Solter, D., Knowles, B. B., Ackerman, S. L. New member of the Snf1/AMPK kinase family, Melk, is expressed in the mouse egg and preimplantation embryo. Molec. Reprod. Dev. 47: 148-156, 1997. 2. Hirose, T., Horvitz, H. R. An Sp1 transcription factor coordinates caspase-dependent and -independent apoptotic pathways. Nature 500: 354-358, 2013. 3. Nagase, T., Seki, N., Ishikawa, K., Tanaka, A., Nomura, N. Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 3: 17-24, 1996.

Uniprot ID: Q14680

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 66 kDa

Calculated Molecular Weight: 23310 MW

Gene ID: 9833

Protein Name: Maternal embryonic leucine zipper kinase

Gene Full Name: maternal embryonic leucine zipper kinase

Synonyms: hMELK; HPK38; KIAA0175; MELK; Protein kinase PK38