Anti-MED13 Antibody Picoband®

SKU: A04545-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: ELISA,Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells
ELISA, 0.1-0.5μg/ml

Gene Name: MED13

Specificity: No cross reactivity with other proteins.

Background: Mediator complex subunit 13 is a protein that in humans is encoded by the MED13 gene. This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Immunogen: E. coli-derived human MED13 recombinant protein (Position: L61-Y240).

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Gustafsson CM, Samuelsson T (Jul 2001). Mediator--a universal complex in transcriptional regulation. Molecular Microbiology. 41 (1): 1–8. 2. Tuchin DA, Erve PR, Schumer W (1976). Prevention of endotoxin--induced irreversible platelet aggregation in vitro. Surgical Forum. 27 (62): 22–3.

Uniprot ID: Q9UHV7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 300 kDa

Calculated Molecular Weight: 34482 MW

Gene ID: 9969

Protein Name: Mediator of RNA polymerase II transcription subunit 13

Gene Full Name: mediator complex subunit 13

Synonyms: mediator complex subunit 13; ARC250; DRIP250; HSPC221; MRD61; THRAP1; TRAP240; MED13