Anti-MCM5 Picoband® Antibody (monoclonal, 4G10)

SKU: M03642-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: MCM5

Specificity:

Background: DNA replication licensing factor MCM5 is a protein that in humans is encoded by the MCM5 gene. It is mapped to 22q12.3. The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

Immunogen: E.coli-derived human MCM5 recombinant protein (Position: K206-H723).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Seroussi, E., Kedra, D., Kost-Alimova, M., Sandberg-Nordqvist, A.-C., Fransson, I., Jacobs, J. F. M., Fu, Y., Pan, H.-Q., Roe, B. A., Imreh, S., Dumanski, J. P. TOM1 genes map to human chromosome 22q13.1 and mouse chromosome 8C1 and encode proteins similar to the endosomal proteins HGS and STAM. Genomics 57: 380-388, 1999. 2. Tsuruga, H., Yabuta, N., Hashizume, K., Ikeda, M., Endo, Y., Nojima, H. Expression, nuclear localization and interactions of human MCM/P1 proteins. Biochem. Biophys. Res. Commun. 236: 118-125, 1997. 3. Vetro, A., Savasta, S., Russo Raucci, A., Cerqua, C., Sartori, G., Limongelli, I., Forlino, A., Maruelli, S., Perucca, P., Vergani, D., Mazzini, G., Mattevi, A., Stivala, L. A., Salviati, L., Zuffardi, O. MCM5: a new actor in the link between DNA replication and Meier-Gorlin syndrome. Europ. J. Hum. Genet. 25: 646-650, 2017.

Uniprot ID: P33992

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2a

Phospho_site:

Clone Number: Clone: 4G10

Observed Molecular Weight: 95 kDa

Calculated Molecular Weight:

Gene ID: 4174

Protein Name: DNA replication licensing factor MCM5

Gene Full Name: minichromosome maintenance complex component 5

Synonyms: CDC46; CDC46 homolog; MCM5; P1 CDC46