Anti-MCC Antibody Picoband®

SKU: A00711-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MCC

Specificity:

Background: MUTATED IN COLORECTAL CANCERS(MCC) is a tumor suppressor gene. It is mapped to 5q22.2. This gene suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. MCC also Inhibits DNA binding of b-catenin/TCF/LEF transcription factors, and it is Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase(PAK) activation. What's more, MCC can interact with SCRIB(via phosphorylated PDZ-binding motif), EZR, SNX27, SLC9A3R1 and SLC9A3R2.

Immunogen: E.coli-derived human MCC recombinant protein (Position: M1-E234).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kinzler, K. W., Nilbert, M. C., Vogelstein, B., Bryan, T. M., Levy, D. B., Smith, K. J., Preisinger, A. C., Hamilton, S. R., Hedge, P., Markham, A., Carlson, M., Joslyn, G., Groden, J., White, R., Miki, Y., Miyoshi, Y., Nishisho, I., Nakamura, Y. Identification of a gene located at chromosome 5q21 that is mutated in colorectal cancers. Science 251: 1366-1370, 1991. 2. MCC, a new interacting protein for Scrib, is required for cell migration in epithelial cells. Arnaud C., Sebbagh M., Nola S., Audebert S., Bidaut G., Hermant A., Gayet O., Dusetti N.J., Ollendorff V., Santoni M.J., Borg J.P., Lecine P. FEBS Lett. 583:2326-2332(2009). 3. The PDZ-binding motif of MCC is phosphorylated at position -1 and controls lamellipodia formation in colon epithelial cells.Pangon L., Van Kralingen C., Abas M., Daly R.J., Musgrove E.A., Kohonen-Corish M.R. Biochim. Biophys. Acta 1823:1058-1067(2012).

Uniprot ID: P23508

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 98 kDa

Calculated Molecular Weight: 42982 MW

Gene ID: 4163

Protein Name: Colorectal mutant cancer protein

Gene Full Name: MCC regulator of Wnt signaling pathway

Synonyms: MCC; MCC1; mutated in colorectal cancers; Protein MCC