Anti-MAP1B Antibody Picoband®

SKU: A01760-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunofluorescence, 5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: MAP1B

Specificity:

Background: This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system.

Immunogen: E.coli-derived human MAP1B recombinant protein (Position: H102-R1849). Human MAP1B shares 89.9% and 89.2% amino acid (aa) sequence identity with mouse and rat MAP1B, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Allen, E., Ding, J., Wang, W., Pramanik, S., Chou, J., Yau, V., Yang, Y. Gigaxonin-controlled degradation of MAP1B light chain is critical to neuronal survival. Nature 438: 224-228, 2005. 2. Cui, L., Zheng, J., Zhao, Q., Chen, J.-R., Liu, H., Peng, G., Wu, Y., Chen, C., He, Q., Shi, H., Yin, S., Friedman, R. A., Chen, Y., Guan, M.-X. Mutations of MAP1B encoding a microtubule-associated phosphoprotein cause sensorineural hearing loss. JCI Insight 5: e136046, 2020. 3. Edelmann, W., Zervas, M., Costello, P., Roback. L., Fischer, I., Hammarback, J. A., Cowan, N., Davies, P., Wainer, B., Kucherlapati, R. Neuronal abnormalities in microtubule-associated protein 1B mutant mice. Proc. Nat. Acad. Sci. 93: 1270-1275, 1996.

Uniprot ID: P46821

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 271 kDa

Calculated Molecular Weight: 271 kDa

Gene ID: 4131

Protein Name: Microtubule-associated protein 1B

Gene Full Name: microtubule associated protein 1B

Synonyms: Microtubule-associated protein 1B; MAP1B heavy chain; MAP-1B; MAP1 light chain LC1