Anti-MAGEL2 Antibody Picoband®

SKU: A06305-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MAGEL2

Specificity:

Background: Prader-Willi syndrome (PWS) is caused by the loss of expression of imprinted genes in chromosome 15q11-q13 region. Affected individuals exhibit neonatal hypotonia, developmental delay, and childhood-onset obesity. Necdin (NDN), a gene involved in the terminal differentiation of neurons, localizes to this region of the genome and has been implicated as one of the genes responsible for the etiology of PWS. This gene is structurally similar to NDN, is also localized to the PWS chromosomal region, and is paternally imprinted, suggesting a possible role for it in PWS.

Immunogen: E.coli-derived human MAGEL2 recombinant protein (Position: Q578-A847).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bayat, A., Bayat, M., Lozoya, R., Schaaf, C. P. Chronic intestinal pseudo-obstruction syndrome and gastrointestinal malrotation in an infant with Schaaf-Yang syndrome--expanding the phenotypic spectrum. Europ. J. Med. Genet. 61: 627-630, 2018. 2. Bischof, J. M., Stewart, C. L., Wevrick, R. Inactivation of the mouse Magel2 gene results in growth abnormalities similar to Prader-Willi syndrome. Hum. Molec. Genet. 16: 2713-2739, 2007. 3. Boccaccio, I., Glatt-Deeley, H., Watrin, F., Roeckel, N., Lalande, M., Muscatelli, F. The human MAGEL2 gene and its mouse homologue are paternally expressed and mapped to the Prader-Willi region. Hum. Molec. Genet. 8: 2497-2505, 1999.

Uniprot ID: Q9UJ55

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 133 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 54551

Protein Name: MAGE-like protein 2

Gene Full Name: MAGE family member L2

Synonyms: MAGEL2; NDNL1; MAGE-like protein 2; Necdin-like protein 1; Protein nM15