Anti-KLHL12 Antibody Picoband® (monoclonal, 2G11D1)

SKU: M08568-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human

Gene Name: KLHL12

Specificity:

Background: Kelch-like protein 12 is a protein that in humans is encoded by the KLHL12 gene. This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human KLHL12 recombinant protein (Position: R27-I331).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 3/7/2012. 2. Jin, L., Pahuja, K. B., Wickliffe, K. E., Gorur, A., Baumgartel, C., Schekman, R., Rape, M. Ubiquitin-dependent regulation of COPII coat size and function. Nature 482: 495-500, 2012. 3. Mai, A., Jung, S.-K., Yonehara, S. hDKIR, a human homologue of the Drosophila kelch protein, involved in a ring-like structure. Exp. Cell Res. 300: 72-83, 2004.

Uniprot ID: Q53G59

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: IgG2a

Phospho_site:

Clone Number: Clone: 2G11D1

Observed Molecular Weight: 63 kDa

Calculated Molecular Weight:

Gene ID: 59349

Protein Name: Kelch-like protein 12

Gene Full Name: kelch like family member 12

Synonyms: C3IP1; CUL3 interacting protein 1; DKIR; Kelch like protein 12; KLHL12