Anti-KDM6B/JMJD3 Picoband® Antibody

SKU: A01309-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Mouse
ELISA, 0.1-0.5μg/ml, -

Gene Name: KDM6B

Specificity: No cross reactivity with other proteins.

Background: Lysine demethylase 6B is a protein that in humans is encoded by the KDM6B gene. It is mapped to 17p13.1. The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Immunogen: E.coli-derived human KDM6B/JMJD3 recombinant protein (Position: R1127-R1643).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Ge, C., Ye, J., Weber, C., Sun, W., Zhang, H., Zhou, Y., Cai, C., Qian, G., Capel, B. The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species. Science 360: 645-648, 2018. 2. Sen, G. L., Webster, D. E., Barragan, D. I., Chang, H. Y., Khavari, P. A. Control of differentiation in a self-renewing mammalian tissue by the histone demethylase JMJD3. Genes Dev. 22: 1865-1870, 2008. 3. Stolerman, E. S., Francisco, E., Stallworth, J. L., Jones, J. R., Monaghan, K. G., Keller-Ramey, J., Person, R., Wentzensen, I. M., McWalter, K., Keren, B., Heron, B., Nava, C., and 19 others. Genetic variants in the KDM6B gene are associated with neurodevelopmental delays and dysmorphic features. Am. J. Med. Genet. 179A: 1276-1286, 2019.

Uniprot ID: O15054

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 177 kDa

Calculated Molecular Weight: 18012 MW

Gene ID: 23135

Protein Name: Lysine-specific demethylase 6B

Gene Full Name: lysine demethylase 6B

Synonyms: lysine demethylase 6B; JMJD3; NEDCFSA; KDM6B; KIAA0346