Anti-JCHAIN Antibody Picoband®

SKU: A02644-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: JCHAIN

Specificity:

Background: Immunoglobulin J chain, also known as IGJ and IGCJ, is a secreted polypeptide which is the first immunoglobulin-related polypeptide expressed during the embryogenesis and differentiation of B cells in the fetal liver. The joining Immunoglobulin J chain is a small polypeptide, expressed by mucosal and glandular plasma cells, which regulates polymer formation of immunoglobulin (Ig)A and IgM. Immunoglobulin J chain / IGJ serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA, it induces larger polymers. Immunoglobulin J chain / IGJ also helps to bind these immunoglobulins to the secretory component. J-chain incorporation into polymeric IgA (pIgA, mainly dimers) and pentameric IgM endows these antibodies with several salient features. Immunoglobulin J chain / IGJ is involved in creating the binding site for pIgR / SC in the Ig polymers, not only by determining the polymeric quaternary structure but also by interacting ly with the receptor protein. Both the immunoglobulin J chain / IGJ and the pIgR/SC are key proteins in secretory immunity.

Immunogen: E.coli-derived human MAPK13 recombinant protein (Position: L328-L365). Human MAPK13 shares 94.7% and 89.5% amino acid (aa) sequence identity with mouse and rat MAPK13, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Koshland, M. E. The coming of age of the immunoglobulin J chain. Annu. Rev. Immun. 3: 425-453, 1985. 2. Lim, J.-H., Cho, S.-J., Park, S.-K., Kim, J., Cho, D., Lee, W. J., Kang, C.-J. Stage-specific expression of two neighboring Crlz1 and IgJ genes during B cell development is regulated by their chromatin accessibility and histone acetylation. J. Immun. 177: 5420-5429, 2006. 3. Max, E. E., McBride, O. W., Morton, C. C., Robinson, M. A. Human J chain gene: chromosomal localization and associated restriction fragment length polymorphisms. Proc. Nat. Acad. Sci. 83: 5592-5596, 1986.

Uniprot ID: P01591

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 23 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 3512

Protein Name: Immunoglobulin J chain

Gene Full Name: joining chain of multimeric IgA and IgM

Synonyms: JCHAIN; IGCJ; IGJ; Immunoglobulin J chain; Joining chain of multimeric IgA and IgM