Anti-Intersectin 1/ITSN1 Antibody Picoband®

SKU: A03616-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ITSN1

Specificity:

Background: Intersectin-1 is a protein that, in humans, is encoded by the ITSN1 gene. The protein encoded by this gene is a cytoplasmic membrane-associated protein that inly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far.

Immunogen: E.coli-derived human Intersectin 1/ITSN1 recombinant protein (Position: A522-P1721).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chen, H., Antonarakis, S. E. The SH3D1A gene maps to human chromosome 21q22.1-q22.2. Cytogenet. Cell Genet. 78: 213-215, 1997. 2. He, G., Wang, H.-R., Huang, S.-K., Huang, C.-L. Intersectin links WNK kinases to endocytosis of ROMK1. J. Clin. Invest. 117: 1078-1087, 2007. 3. Irie, F., Yamaguchi, Y. EphB receptors regulate dendritic spine development via intersectin, Cdc42 and N-WASP. Nature Neurosci. 5: 1117-1118, 2002.

Uniprot ID: Q15811

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 150 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 6453

Protein Name: Intersectin-1

Gene Full Name: intersectin 1

Synonyms: Intersectin 1; ITSN; ITSN1; SH3D1A; SH3P17