Anti-Interferon gamma IFNG Antibody (monoclonal, 8E9)

SKU: M00393-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Rat

Applications: ELISA (Cap)

Application Details: ELISA (Cap), 1-5μg/ml

Gene Name: IFNG

Specificity: No cross reactivity with other proteins.

Background: Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Immunogen: E. coli-derived rat Interferon gamma recombinant protein (Position: Q23-C156). Rat Interferon gamma shares 38% and 86% amino acid (aa) sequence identity with human and mouse Interferon gamma, respectively.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Nanba D, Higashiyama S (February 2004). Dual intracellular signaling by proteolytic cleavage of membrane-anchored heparin-binding EGF-like growth factor. Cytokine Growth Factor Rev. 15 (1): 13–9. 2. Jin K, Mao XO, Sun Y, Xie L, Jin L, Nishi E, Klagsbrun M, Greenberg DA (July 2002). Heparin-binding epidermal growth factor-like growth factor: hypoxia-inducible expression in vitro and stimulation of neurogenesis in vitro and in vivo. J. Neurosci. 22 (13): 5365–73. 3. Fen, Z., Dhadly, M. S., Yoshizumi, M., Hilkert, R. J., Quertermous, T., Eddy, R. L., Shows, T. B., Lee, M.-E. Structural organization and chromosomal assignment of the gene encoding the human heparin-binding epidermal growth factor-like growth factor/diphtheria toxin receptor. Biochemistry 32: 7932-7938, 1993.

Uniprot ID: P01581

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: Clone: 8E9

Observed Molecular Weight: 53 kDa

Calculated Molecular Weight:

Gene ID: 25712

Protein Name: Interferon gamma

Gene Full Name: interferon gamma

Synonyms: IFG; IFI; IFN gamma; IFN γ; IFNG; IFN-gamma; IFN-γ