Anti-IKB alpha/NFKBIA Antibody Picoband®

SKU: PB9291

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: NFKBIA

Specificity: No cross reactivity with other proteins.

Background: NFKBIA, also called IKBA or MAD-3, is one member of a family of cellular proteins that function to inhibit the NF-κB transcription factor. It is mapped to 14q13.2. NFKBIA inhibits NF-κB by masking the nuclear localization signals (NLS) of NF-κB proteins and keeping them sequestered in an inactive state in the cytoplasm. It moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. The effect of the nonpathogenic bacteria is specific to the SCF complex substrates CTNNB1 and NFKBIA. This may help to explain the beneficial effects of treatment of inflammatory bowel disease with nonpathogenic probiotic enteric organisms. In addition, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-κB's proper functioning.

Immunogen: E.coli-derived human IKB alpha recombinant protein (Position: Q3-Q112). Human IKB alpha shares 87% and 86% amino acid (aa) sequence identity with mouse and rat IKB alpha, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Jacobs MD, Harrison SC (1998). Structure of an IkappaBalpha/NF-kappaB complex. Cell 95 (6): 749–58. 2. Neish, A. S.., Gewirtz, A. T., Zeng, H., Young, A. N., Hobert, M. E., Karmali, V., Rao, A. S., Madara, J. L. Prokaryotic regulation of epithelial responses by inhibition of I-kappa-B-alpha ubiquitination. Science 289: 1560-1563, 2000. 3. Verma IM, Stevenson JK, Schwarz EM, Van Antwerp D, Miyamoto S (1995). Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev.9 (22): 2723–35.

Uniprot ID: P25963

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 39 kDa

Calculated Molecular Weight: 36 kDa

Gene ID: 4792

Protein Name: NF-kappa-B inhibitor alpha

Gene Full Name: NFKB inhibitor alpha

Synonyms: I kappa B alpha; IkappaBalpha; IkB alpha; IKBA; Ikβ-α; MAD 3; MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA