Anti-IFT88 Antibody Picoband®

SKU: A06814-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: IFT88

Specificity: No cross reactivity with other proteins.

Background: Intraflagellar transport protein 88 homolog is a protein that in humans is encoded by the IFT88 gene. It is mapped to 13q12.11. This gene encodes a member of the tetratrico peptide repeat (TPR) family. The encoded protein is involved in cilium biogenesis. Mutations of a similar gene in mouse can cause polycystic kidney disease. Several transcript variants encoding distinct isoforms have been identified for this gene.

Immunogen: E.coli-derived human IFT88 recombinant protein (Position: E198-K814).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Cano, D. A., Murcia, N. S., Pazour, G. J., Hebrok, M. orpk mouse model of polycystic kidney disease reveals essential role of primary cilia in pancreatic tissue organization. Development 131: 3457-3467, 2004. 2. Delaval, B., Bright, A., Lawson, N. D., Doxsey, S. The cilia protein IFT88 is required for spindle orientation in mitosis. Nature Cell Biol. 13: 461-468, 2011. 3. Huangfu, D., Liu, A., Rakeman, A. S., Murcia, N. S., Niswander, L., Anderson, K. V. Hedgehog signalling in the mouse requires intraflagellar transport proteins. Nature 426: 83-87, 2003.

Uniprot ID: Q13099

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 94 kDa

Calculated Molecular Weight: 104553 MW

Gene ID: 8100

Protein Name: Intraflagellar transport protein 88 homolog

Gene Full Name: intraflagellar transport 88

Synonyms: IFT88; TPR repeat protein 10; TTC10