Anti-IDH3B Antibody Picoband®

SKU: A09433-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IP,IHC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: IDH3B

Specificity:

Background: Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial is an enzyme that in humans is encoded by the IDH3B gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Immunogen: E.coli-derived human IDH3B recombinant protein (Position: A32-D362). Human IDH3B shares 97% amino acid (aa) sequence identity with rat IDH3B.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartong, D. T., Dange, M., McGee, T. L., Berson, E. L., Dryja, T. P., Colman, R. F. Insights from retinitis pigmentosa into the roles of isocitrate dehydrogenases in the Krebs cycle. Nature Genet. 40: 1230-1234, 2008. 2. Kim, Y.-O., Park, S.-H., Kang, Y.-J., Koh, H.-J., Kim, S.-H., Park, S.-Y., Sohn, U., Huh, T.-L. Assignment of mitochondrial NAD(+)-specific isocitrate dehydrogenase beta subunit gene (IDH3B) to human chromosome band 20p13 by in situ hybridization and radiation hybrid mapping. Cytogenet. Cell Genet. 86: 240-241, 1999. 3. Zenteno, J. C., Garcia-Montano, L. A., Cruz-Aguilar, M., Ronquillo, J., Rodas-Serrano, A., Aguilar-Castul, L., Matsui, R., Vencedor-Meraz, C. I., Arce-Gonzalez, R., Graue-Wiechers, F., Gutierrez-Paz, M., Urrea-Victoria, T., de Dios Cuadras, U., Chacon-Camacho, O. F. Extensive genic and allelic heterogeneity underlying inherited retinal dystrophies in Mexican patients molecularly analyzed by next-generation sequencing. Molec. Genet. Genomic Med. 8: e1044, 2020. Note: Electronic Article.

Uniprot ID: O43837

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 42 kDa

Calculated Molecular Weight:

Gene ID: 3420

Protein Name: Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial

Gene Full Name: isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit beta

Synonyms: FLJ11043; H IDHB; IDH3B; RP46