Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22)

SKU: MA1050

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Bovine,Human,Mouse,Rabbit,Rat

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, bovine, rabbit, rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, -
Western blot, 1μg/ml, Human, bovine, mouse, rabbit, rat

Gene Name: HSPA1A; HSPA1B

Specificity: No cross reactivity with other proteins.

Background: Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.

Immunogen: HSP70 isolated from bovine brain.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Milner, C. M.; Campbell, R. D. : Structure and expression of the three MHC-linked HSP70 genes. Immunogenetics 32: 242-251, 1990. 2. Ungewickell, E. : The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles. EMBO J. 4: 3385-3391, 1985.

Uniprot ID: P17879

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: BRM-22

Observed Molecular Weight: 70 kDa

Calculated Molecular Weight: 70176 MW

Gene ID: Human(3303), Mouse(193740), Rat(24472), Human(3304), Mouse(15511), Rat(294254)

Protein Name: aldehyde dehydrogenase (NAD(+))

Gene Full Name: heat shock protein 1B

Synonyms: HSPA1; Hsp70-1; Hsp70-2; Heat shock 70 kDa protein 1/2