Anti-HSD3B1 Antibody Picoband®

SKU: A02856-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: HSD3B1

Specificity: No cross reactivity with other proteins.

Background: HSD3B1 is a human gene that encodes for a 3beta-hydroxysteroid dehydrogenase/delta (5)-delta (4)isomerase type I or hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1. It is mapped to 1p12. The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.

Immunogen: E.coli-derived human HSD3B1 recombinant protein (Position: Q17-Q373).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Taneja SS (January 2017). Re: HSD3B1 and Resistance to Androgen-Deprivation Therapy in Prostate Cancer: A Retrospective, Multicohort Study. The Journal of Urology. 197 (1): 150. 2. Hearn, Jason (October 2016). HSD3B1 and resistance to androgen-deprivation therapy in prostate cancer: a retrospective, multicohort study. Lancet Oncology. 17 (10): 1435–1444. 3. Russell AJ, Gaffney D, Gardiner MT, Nickson DA, Sutcliffe RG (March 1991). Synonymous polymorphism in the coding sequence of human 3-beta hydroxysteroid-5-ene dehydrogenase (HSD). Nucleic Acids Research. 19 (5): 1172.

Uniprot ID: P14060

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 42 kDa

Calculated Molecular Weight: 36043 MW

Gene ID: 3283

Protein Name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Gene Full Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1

Synonyms: hydroxy-delta-5-steroid dehydrogenase; 3 beta- and steroid delta-isomerase 1; 3BETAHSD; HSD3B; HSDB3; HSDB3A; SDR11E1; HSD3B1