Anti-HMGCR Antibody Picoband®

SKU: A00643-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: HMGCR

Specificity:

Background: HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human HMGCR recombinant protein (Position: H268-V842).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aulchenko, Y. S., Ripatti, S., Lindqvist, I., Boomsma, D., Heid, I. M., Pramstaller, P. P., Penninx, B. W. J. H., Janssens, A. C. J. W., Wilson, W. F., Spector, T., Martin, N. G., Pedersen, N. L. {and 45 others}: Loci influencing lipid levels and coronary heart disease risk in 16 European population cohorts. Nature Genet. 41: 47-55, 2009. 2. Chasman, D. I., Posada, D., Subrahmanyan, L., Cook, N. R., Stanton, V. P., Jr., Ridker, P. M. Pharmacogenetic study of statin therapy and cholesterol reduction. JAMA 291: 2821-2827, 2004. 3. Goldstein, J. L., Brown, M. S. Regulation of the mevalonate pathway. Nature 343: 425-430, 1990.

Uniprot ID: P04035

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 97 kDa

Calculated Molecular Weight:

Gene ID: 3156

Protein Name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase

Gene Full Name: 3-hydroxy-3-methylglutaryl-CoA reductase

Synonyms: HMG CoA reductase; HMGCR