Anti-HLA-DR/HLA-DRA Antibody Picoband®

SKU: A01195

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IHC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: HLA-DRA

Specificity: No cross reactivity with other proteins.

Background: HLA class II histocompatibility antigen, DR alpha chain?is a?protein?that in humans is encoded by the HLA-DRA?gene. It is mapped to 6p21.32. HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

Immunogen: E.coli-derived human HLA-DR/HLA-DRA recombinant protein (Position: I26-L254).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Tjernlund U, Scheynius A, Johansson C, et al. (1989). T-cell response to purified protein derivative after removal of Langerhans' cells from epidermal cell suspensions containing keratinocytes expressing class II transplantation antigens. Scand. J. Immunol. 28 (6): 667–73. 2. Chelladurai, M., Honn, K. V., Walz, D. A. HLA-DR is a procoagulant. Biochem. Biophys. Res. Commun. 178: 467-473, 1991. 3. Das, H. K., Lawrance, S. K., Weissman, S. M. Structure and nucleotide sequence of the heavy chain gene of HLA-DR. Proc. Nat. Acad. Sci. 80: 3543-3547, 1983.

Uniprot ID: P01903

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 37 kDa

Calculated Molecular Weight: 82536 MW

Gene ID: 3122

Protein Name: HLA class II histocompatibility antigen, DR alpha chain

Gene Full Name: major histocompatibility complex, class II, DR alpha

Synonyms: HLA DRA; HLA DRA1; HLA-DR; HLA-DRA; MHC class II antigen DRA