Anti-HK3 Antibody Picoband®

SKU: A05145-3

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: HK3

Specificity:

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.

Immunogen: E.coli-derived human HK3 recombinant protein (Position: M1-K789). Human HK3shares 84.7% and 84.8% amino acid (aa) sequence identity with mouse and rat HK3, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Colosimo, A., Calabrese, G., Gennarelli, M., Ruzzo, A. M., Sangiuolo, F., Magnani, M., Palka, G., Novelli, G., Dallapiccola, B. Assignment of the hexokinase type 3 gene (HK3) to human chromosome band 5q35.3 by somatic cell hybrids and in situ hybridization. Cytogenet. Cell Genet. 74: 187-188, 1996. 2. Furuta, H., Nishi, S., Le Beau, M. M., Fernald, A. A., Yano, H., Bell, G. I. Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization. Genomics 36: 206-209, 1996. 3. Harris, H., Hopkinson, D. A. Average heterozygosity per locus in man: an estimate based on the incidence of enzyme polymorphism. Ann. Hum. Genet. 36: 9-20, 1972.

Uniprot ID: P52790

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 99 kDa

Calculated Molecular Weight: 99 kDa

Gene ID: 3101

Protein Name: Hexokinase-3

Gene Full Name: hexokinase 3

Synonyms: Hexokinase 3; hexokinase 3 (white cell); Hexokinase type III; HK III; HK3; HKIII; HXK3