Anti-HIP2/LIG/UBE2K Antibody Picoband®

SKU: A05895-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat,Monkey

Applications: ELISA,Flow Cytometry,IP,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat, Monkey
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: UBE2K

Specificity:

Background: The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene.

Immunogen: E.coli-derived human HIP2/LIG/UBE2K recombinant protein (Position: D33-E195).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kalchman, M. A., Graham, R. K., Xia, G., Koide, H. B., Hodgson, J. G., Graham, K. C., Goldberg, Y. P., Gietz, R. D., Pickart, C. M., Hayden, M. R. Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme. J. Biol. Chem. 271: 19385-19394, 1996.

Uniprot ID: P61086

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 22 kDa

Calculated Molecular Weight:

Gene ID: 3093

Protein Name: Ubiquitin-conjugating enzyme E2 K

Gene Full Name: ubiquitin conjugating enzyme E2 K

Synonyms: E2-25K; HIP 2; HIP2; HYPG; LIG; UBE2K; Ubiquitin carrier protein; Ubiquitin protein ligase