Anti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7)

SKU: M01504-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IF,IHC,IHC-F,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunofluorescence, 2μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: HK1

Specificity: No cross reactivity with other proteins.

Background: Hexokinase-1 (HK1) is an enzyme that in humans is encoded by the HK1 gene on chromosome 10. It is mapped to 10q22.1. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

Immunogen: E.coli-derived human Hexokinase 1/HK1 recombinant protein (Position: D17-R323).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Andreoni, F., Ruzzo, A., Magnani, M. Structure of the 5-prime region of the human hexokinase type I (HKI) gene and identification of an additional testis-specific HKI mRNA. Biochim. Biophys. Acta 1493: 19-26, 2000. 2. Bianchi, M., Crinelli, R., Serafini, G. Giammarini, C., Magnani, M. Molecular bases of hexokinase deficiency. Biochim. Biophys. Acta 1360: 211-221, 1997. 3. Bianchi, M., Magnani, M. Hexokinase mutations that produce nonspherocytic hemolytic anemia. Blood Cells Mol. Dis. 21: 2-8, 1995.

Uniprot ID: P19367

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2a

Phospho_site:

Clone Number: Clone: 4B7

Observed Molecular Weight: 120 kDa

Calculated Molecular Weight:

Gene ID: 3098

Protein Name: Hexokinase-1

Gene Full Name: hexokinase 1

Synonyms: Brain form hexokinase; Cell and organelle markers; Hexokinase 1; Hexokinase type I; HK I; HK1; HK1 ta; HK1 tb; HK1 tc; HKI; HXK1