Anti-HERC5 Antibody Picoband®

SKU: A05285-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,IF,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
ELISA, 0.1-0.5μg/ml

Gene Name: HERC5

Specificity: No cross reactivity with other proteins.

Background: Probable E3 ubiquitin-protein ligase HERC5 is an enzyme that in humans is encoded by the HERC5 gene. It is mapped to 4q22.1. This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets.

Immunogen: E.coli-derived human HERC5 recombinant protein (Position: F793-G1024).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Hochrainer, K., Mayer, H., Baranyi, U., Binder, B. R., Lipp, J., Kroismayr, R. The human HERC family of ubiquitin ligases: novel members, genomic organization, expression profiling, and evolutionary aspects. Genomics 85: 153-164, 2005. 2. Mitsui, K., Nakanishi, M., Ohtsuka, S., Norwood, T. H., Okabayashi, K., Miyamoto, C., Tanaka, K., Yoshimura, A., Ohtsubo, M. A novel human gene encoding HECT domain and RCC1-like repeats interacts with cyclins and is potentially regulated by the tumor suppressor proteins. Biochem. Biophys. Res. Commun. 266: 115-122, 1999. 3. Wong, J. J. Y., Pung, Y. F., Sze, N. S.-K., Chin, K.-C. HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets. Proc. Nat. Acad. Sci. 103: 10735-10740, 2006.

Uniprot ID: Q9UII4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 117 kDa

Calculated Molecular Weight: 39411 MW

Gene ID: 51191

Protein Name: E3 ISG15--protein ligase HERC5

Gene Full Name: HECT and RLD domain containing E3 ubiquitin protein ligase 5

Synonyms: CEB1; CEBP1; Cyclin E binding protein 1; E3 ISG15 protein ligase HERC5; hect domain and RLD 5; HERC5