Anti-Hepsin/HPN Antibody Picoband®

SKU: A02350-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: HPN

Specificity:

Background: This gene encodes a type II transmembrane serine protease that may be involved in diverse cellular functions, including blood coagulation and the maintenance of cell morphology. Expression of the encoded protein is associated with the growth and progression of cancers, particularly prostate cancer. The protein is cleaved into a catalytic serine protease chain and a non-catalytic scavenger receptor cysteine-rich chain, which associate via a single disulfide bond. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human Hepsin/HPN recombinant protein (Position: D47-L417). Human Hepsin/HPNshares 90% and 89.2% amino acid (aa) sequence identity with mouse and rat Hepsin/HPN, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dhanasekaran, S. M., Barrette, T. R., Ghosh, D., Shah, R., Varambally, S., Kurachi, K., Pienta, K. J., Rubin, M. A., Chinnaiyan, A. M. Delineation of prognostic biomarkers in prostate cancer. Nature 412: 822-826, 2001. 2. Guipponi, M., Toh, M.-Y., Tan, J., Park, D., Hanson, K., Ballana, E., Kwong, D., Cannon, P. Z. F., Wu, Q., Gout, A., Delorenzi, M., Speed, T. P., Smith, R. J. H., Dahl, H. H., Petersen, M., Teasdale, R. D., Estivill, X., Park, W. J., Scott, H. S. An integrated genetic and functional analysis of the role of type II transmembrane serine proteases (TMPRSSs) in hearing loss. Hum. Mutat. 29: 130-141, 2008. 3. Leytus, S. P., Loeb, K. R., Hagen, F. S., Kurachi, K., Davie, E. W. A novel trypsin-like serine protease (hepsin) with a putative transmembrane domain expressed by human liver and hepatoma cells. Biochemistry 27: 1067-1074, 1988.

Uniprot ID: P05981

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 51 kDa

Calculated Molecular Weight: 45 kDa

Gene ID: 3249

Protein Name: Serine protease hepsin

Gene Full Name: hepsin

Synonyms: AA408042; AI788568; hepsin; Hpn