Anti-Heparanase 1/Hpse Antibody Picoband®

SKU: A01313

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Mouse

Gene Name: Hpse

Specificity: No cross reactivity with other proteins.

Background: Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate and has been implicated in inflammation and tumor angiogenesis and metastasis. The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential corresponds with increased heparanase activity for a number of cell lines.

Immunogen: E.coli-derived mouse Heparanase 1 recombinant protein (Position: K206-K457). Mouse Heparanase 1 shares 80.2% and 96.4% amino acid (aa) sequence identity with human and rat Heparanase 1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Hulett MD, Freeman C, Hamdorf BJ, Baker RT, Harris MJ, Parish CR (July 1999), Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis, Nature medicine 5 (7): 803–9. 2. Nakajima M, Irimura T, Nicolson GL. (1988), Heparanases and tumor metastasis, J. Cell. Biochem. 36 (2): 157–167. 3. Toyoshima, M.; Nakajima, M. : Human heparanase: purification, characterization, cloning, and expression. J. Biol. Chem. 274: 24153-24160, 1999. 4. Vlodavsky I, Friedmann Y, Elkin M, Aingorn H, Atzmon R, Ishai-Michaeli R, Bitan M, Pappo O, Peretz T, Michal I, Spector L, Pecker I (July 1999), Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis, Nature medicine 5 (7): 793–802. 5. Vlodavsky I, Goldshmidt O, Zcharia E, et al. (2003), Mammalian heparanase: involvement in cancer metastasis, angiogenesis and normal development., Semin. Cancer Biol. 12 (2): 121–9.

Uniprot ID: Q6YGZ1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 65 kDa

Calculated Molecular Weight: 60066 MW

Gene ID: 15442

Protein Name: Heparanase

Gene Full Name: heparanase

Synonyms: Endo glucoronidase; HEP; heparanase; Heparanase 1; HPA; Hpa1; HPR1; HPSE; HPSE1; HSE1