Anti-HECTD1 Antibody Picoband®

SKU: A08428-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: HECTD1

Specificity:

Background: HECTD1 (E3 ubiquitin-protein ligase), which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then ly transfers the ubiquitin to targeted substrates,may be required for development of the head mesenchyme and neural tube closure.It is also involved in sporadic Parkinson's disease(PMID:16714300).This protein is specific to HECTD1.

Immunogen: E.coli-derived human HECTD1 recombinant protein (Position: D39-S566).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aleidi, S. M., Yang, A., Sharpe, L. J., Rao, G., Cochran, B. J., Rye, K.-A., Kockx, M., Brown, A. J., Gelissen, I. C. The E3 ubiquitin ligase, HECTD1, is involved in ABCA1-mediated cholesterol export from macrophages. Biochim. Biophys. Acta Molec. Cell Biol. Lipids 1863: 359-368, 2018. 2. D'Alonzo, D., Emch, F. H., Shen, X., Bruder, E., De Geyter, C., Zhang, H. Hectd1 is essential for embryogenesis in mice. Gene Expr. Patterns 34: 119064, 2019. Note: Electronic Article. 3. Gross, M. B. Personal Communication. Baltimore, Md. 10/29/2019.

Uniprot ID: Q9ULT8

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 290 kDa

Calculated Molecular Weight: 82457 MW

Gene ID: 25831

Protein Name: E3 ubiquitin-protein ligase HECTD1

Gene Full Name: HECT domain E3 ubiquitin protein ligase 1

Synonyms: E3 ligase for inhibin receptor; EULIR; HECT domain containing 1; HECTD1; KIAA1131