Anti-HAUSP/USP7 Antibody Picoband®

SKU: A01239-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells
ELISA, 0.1-0.5μg/ml

Gene Name: USP7

Specificity: No cross reactivity with other proteins.

Background: Ubiquitin-specific-processing protease 7 (USP7), also known as ubiquitin carboxyl-terminal hydrolase 7 or herpesvirus-associated ubiquitin-specific protease (HAUSP), is an enzyme that in humans is encoded by the USP7 gene. The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder.

Immunogen: E. coli-derived human HAUSP/USP7 recombinant protein (Position: L258-D483).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: USP7 ubiquitin specific peptidase 7 (herpes virus-associated). 2. Holowaty MN, Sheng Y, Nguyen T, Arrowsmith C, Frappier L (Nov 2003). Protein interaction domains of the ubiquitin-specific protease, USP7/HAUSP. The Journal of Biological Chemistry. 278 (48): 47753–61. 3. Li M, Chen D, Shiloh A, Luo J, Nikolaev AY, Qin J, Gu W (Apr 2002). Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization. Nature. 416 (6881): 648–53.

Uniprot ID: Q93009

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 140 kDa

Calculated Molecular Weight:

Gene ID: 7874

Protein Name: Ubiquitin carboxyl-terminal hydrolase 7

Gene Full Name: ubiquitin specific peptidase 7

Synonyms: Deubiquitinating enzyme 7; HAUSP; TEF1; Ubiquitin thioesterase 7; USP7