Anti-HAND1 Antibody Picoband®

SKU: A06496-3

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human

Gene Name: HAND1

Specificity:

Background: The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HAND1. Human HAND1 shares 90% and 93.8% amino acid (aa) sequence identity with mouse and rat HAND1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Cserjesi, P., Brown, D., Lyons, G. E., Olson, E. N. Expression of the novel basic helix-loop-helix gene eHAND in neural crest derivatives and extraembryonic membranes during mouse development. Dev. Biol. 170: 664-678, 1995. 2. Firulli, A. B., McFadden, D. G., Lin, Q., Srivastava, D., Olson, E. N. Heart and extra-embryonic mesodermal defects in mouse embryos lacking the bHLH transcription factor Hand1. Nature Genet. 18: 266-270, 1998. 3. Knofler, M., Meinhardt, G., Bauer, S., Loregger, T., Vasicek, R., Bloor, D. J., Kimber, S. J., Husslein, P. Human Hand1 basic helix-loop-helix (bHLH) protein: extra-embryonic expression pattern, interaction partners and identification of its transcriptional repressor domains. Biochem J. 361: 641-651, 2002.

Uniprot ID: O96004

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 24 kDa

Calculated Molecular Weight: 24 kDa

Gene ID: 9421

Protein Name: Heart- and neural crest derivatives-expressed protein 1

Gene Full Name: heart and neural crest derivatives expressed 1

Synonyms: HAND1; BHLHA27; EHAND; Heart- and neural crest derivatives-expressed protein 1; Class A basic helix-loop-helix protein 27; bHLHa27; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1; eHAND