Anti-H2AFY/MACROH2A1 Antibody Picoband®

SKU: A04635-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MACROH2A1

Specificity:

Background: Core histone macro-H2A.1 is a protein that in humans is encoded by the H2AFY gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen: E.coli-derived human H2AFY/MACROH2A1 recombinant protein (Position: A180-K301). Human MACROH2A1 shares 97.5% amino acid (aa) sequence identity with both mouse and rat MACROH2A1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chakravarthy, S., Gundimella, S. K. Y., Caron, C., Perche, P.-Y., Pehrson, J. R., Khochbin, S., Luger, K. Structural characterization of the histone variant macroH2A. Molec. Cell. Biol. 25: 7616-7624, 2005. 2. Kapoor, A., Goldberg, M. S., Cumberland, L. K., Ratnakumar, K., Segura, M. F., Emanuel, P. O., Menendez, S., Vardabasso, C., LeRoy, G., Vidal, C. I., Polsky, D., Osman, I., Garcia, B. A., Hernando, E., Bernstein, E. The histone variant macroH2A suppresses melanoma progression through regulation of CDK8. Nature 468: 1105-1109, 2010. 3. Kragesteen, B. K., Brancati, F., Digilio, M. C., Mundlos, S., Spielmann, M. H2AFY promoter deletion causes PITX1 endoactivation and Liebenberg syndrome. J. Med. Genet. 56: 246-251, 2019.

Uniprot ID: O75367

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 39 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 9555

Protein Name: Core histone macro-H2A.1

Gene Full Name: macroH2A.1 histone

Synonyms: Core histone macro H2A.1; H2A histone family; member Y; H2A.y; H2A/y; H2AF12M; H2AFJ; H2AFY; Histone H2A.y; Histone macroH2A1; MACROH2A1; MACROH2A1.1; macroH2A1.2; mH2A1