Anti-H1-7 Antibody Picoband®

SKU: A13834-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: H1-7

Specificity:

Background: H1 histone family, member N, testis-specific is a member of the histone family of nuclear proteins which are a component of chromatin. In humans, this protein is encoded by the H1FNT gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. The related mouse gene encodes a testis specific protein that is required for spermatogenesis and male fertility.

Immunogen: E.coli-derived human H1-7 recombinant protein (Position: H35-R130). Human H1-7 shares 61.3% and 64.9 amino acid (aa) sequence identity with mouse and rat H1-7, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Martianov, I., Brancorsini, S., Catena, R., Gansmuller, A., Kotaja, N., Parvinen, M., Sassone-Corsi, P., Davidson, I. Polar nuclear localization of H1T2, a histone H1 variant, required for spermatid elongation and DNA condensation during spermiogenesis. Proc. Nat. Acad. Sci. 102: 2808-2813, 2005. 2. Tanaka, H., Iguchi, N., Isotani, A., Kitamura, K., Toyama, Y., Matsuoka, Y., Onishi, M., Masai, K., Maekawa, M., Toshimori, K., Okabe, M., Nishimune, Y. HANP1/H1T2, a novel histone H1-like protein involved in nuclear formation and sperm fertility. Molec. Cell. Biol. 25: 7107-7119, 2005. 3. Tanaka, H., Matsuoka, Y., Onishi, M., Kitamura, K., Miyagawa, Y., Nishimura, H., Tsujimura, A., Okuyama, A., Nishimune, Y. Expression profiles and single-nucleotide polymorphism analysis of human HANP1/H1T2 encoding a histone H1-like protein. Int. J. Androl. 29: 353-359, 2006.

Uniprot ID: Q75WM6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 28 kDa

Calculated Molecular Weight: 28 kDa

Gene ID: 341567

Protein Name: Testis-specific H1 histone

Gene Full Name: H1.7 linker histone

Synonyms: H1FNT; HANP1; Testis-specific H1 histone; Haploid germ cell-specific nuclear protein 1; Histone H1t2